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pet28a tfam expression vector  (Addgene inc)


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    Addgene inc pet28a tfam expression vector
    Pet28a Tfam Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet28a tfam expression vector/product/Addgene inc
    Average 94 stars, based on 11 article reviews
    pet28a tfam expression vector - by Bioz Stars, 2026-05
    94/100 stars

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    pet 28a expression vector  (Addgene inc)
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    (A) In silico cloning of the vaccine construct sequence into the <t>pET-28a</t> (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.
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    (A) In silico cloning of the vaccine construct sequence into the pET-28a (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.

    Journal: Journal of Immunology Research

    Article Title: In Silico Design and Characterization of a Multiepitope Vaccine Candidate Against Brucella canis Using a Reverse Vaccinology Approach

    doi: 10.1155/jimr/6348238

    Figure Lengend Snippet: (A) In silico cloning of the vaccine construct sequence into the pET-28a (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.

    Article Snippet: The pET-28a (+) expression vector, obtained from the Addgene database ( https://www.addgene.org/ ) [ ], was selected as the cloning vector.

    Techniques: In Silico, Cloning, Construct, Sequencing, Expressing, Plasmid Preparation, Clone Assay, Agarose Gel Electrophoresis, Molecular Weight, Recombinant